Jack Murphy

A Biomedical Eningeering Student
at Trinity College Dublin

Background

I was born and raised in New York City. While in high school I had the opportunity to conduct research in the Costa Lab at the Icahn School of Medicine at Mount Sinai. Through this experience, I developed a deep interest in the fields of tissue engineering and cardiac regeneration. I have now moved my passions over to Dublin while I pursue a degree in Biomedical Engineering from Trinity College Dublin.

During term, I work as lab assistant in the Monaghan Lab in the Trinity Biomedical Sciences Institute. While, during the summer I spend my time as a lab assistant in the Costa Lab at Mt. Sinai.

Have a look at my previous work throughout this website and feel free to get in touch.

Monaghan Lab 2018/2019

Research Experience

Costa Lab at the Icahn School of Medicine

New York City, USA

Beating Monolayer of Cardiomyocytes

Cell Culture

• Maintained, and expanded,

induced-Pluripotent Stem Cells (iPSCs)
Mesenchymal Stem Cells (MSCs)
Cardiac Progenitor Cells (CPCs)

• Differentiated iPSCs into cardiomyocytes

6 Engineered Tisues Inverted

Tissue Fabrication

• Fabricated 3-D human engineered cardiac tissues from iPSC-derived cardiomyocytes.
• Supplemented specific tissues with MSCs or CPCs depending on effect being studied.

Beating Engineered Tissue

Contractility Analysis

Video Adapted from Turnbull at al. The FASEB Journal (2014).

• Used LabView to capture both the spontaneous and electrically stimulated contractions of the tissues.
• Analyzed the tissue function using MatLab.

3-D Printed Stand

3-D Design and Printing

• Designed and printed 3-D accessories to help simplify and streamline the data collection process.

Monaghan Lab at Trinity College Dublin

Dublin, Ireland

Goal

The goal of this study is to analyze the fibrotic capsule that forms around silicone devices placed under the skin.

More information to come.

Dean Lab at Columbia University

New York City, USA

Worked in the Dean Lab at Columbia University focused on creating a graphene device insulated by a layer of boron nitride on both sides. If successful it would make it much easier to study how current flows through superconductors.

Was able to exfoliate graphite to get a monolayer of graphene.

Used an atomic force microscope (AFM) to image the graphene and device in search of imperfections and extraneous particles.

Publications

2019

Murphy, J. F., Mayourian, J., Stillitano, F., Munawar, S., Broughton, K. M., Agullo-Pascual, E., Sussman, M. A., Hajjar, R. J., Costa, K. D. & Turnbull, I. C. (2019). Adult human cardiac stem cell supplementation effectively increases contractile function and maturation in human engineered cardiac tissues. Stem Cell Res Ther 10, 373

[Abstract] Murphy, J. F., Mayourian, J., Stillitano, F., Munawar, S., Broughton, K. M., Sussman, M. A., Hajjar, R. J., Costa, K. D. & Turnbull, I. C. (2019). Cardiac Stem Cells from Failing Heart Improve Contractility of Engineered Human Myocardium. 2019 Biomedical Engineering Society Annual Meeting. Philadelphia, PA. October 16-19, 2019

[Abstract] Salazar, S. I., Mayourian, J., Chionuma, H., Murphy, J., Costa, K. D. & Turnbull, I. C. (2019). Comparison of cell culturing conditions and extracellular vesicle preservation techniques to maximize cardioactive potency of the adult stem cell sectretome. Extracellular Vesicales in Diagnostics and Therapeutics Symposium. New York Academy of Sciences. February 19, 2019. Poster Presentation.

2018

[Abstract] Murphy, J. F., Mayourian, J., Salazar, S. I., Ceholski, D. K., Pothula, V. M., Stillitano, F., Dave, J., Broughton, K., Sussman, M., Hajjar, R. J., Costa, K. D. & Turnbull, I. C. (2018). Adult Human Cardiac Progenitor Cells Enhance Contractile Force in Human Engineered Cardiac Tissues. AHA Scientific Sessions 2018. Nov 12, 2018.

[Abstract] Turnbull, I. C., Murphy, J. F., Mayourian, J., Salazar, S. I., Ceholski, D. K., Pothula, V., Stillitano, F., Broughton, K., Sussman, M., Hajjar, R. J. & Costa, K. D. (2018). Heterocellular Coupling Mediates Pro-Contractile Effects Of Cardiac Progenitor Cells In Human Engineered Cardiac Tissue. International Society for Stem Cell Research. June 2018: 52-53

Turnbull, I. C., Mayourian, J., Murphy, J. F., Stillitano, F., Ceholski, D. K. & Costa, K. D. (2018). Cardiac Tissue Engineering Models of Inherited and Acquired Cardiomyopathies. Methods Mol Biol 1816: 145-159

Mayourian, J., Ceholski, D. K., Gorski, P. A., Mathiyalagan, P., Murphy, J. F., Salazar, S. I., Stillitano, F., Hare, J. M., Sahoo, S., Hajjar, R. J. & Costa, K. D. (2018). Exosomal microRNA-21-5p Mediates Mesenchymal Stem Cell Paracrine Effects on Human Cardiac Tissue Contractility. Circ Res 122(7): 933-944.

Education

Biomedical Engineering (2018-Present) Expected Graduation 2023 (B.A.I + M.A.I)

High School for Mathemeatics, Science, and Engineering

New York Regents Diploma with Honors (2014-2018) Mt. Sinai Specialization

Other Interests

Rock Climbing

Rock Climber

Rock Climbing is one of my favorite pastimes, I spend most weekends at the climbing gym or climbing outside.

I also like to use climbing as an excuse to travel almost every school break.

The Gunks, New York

Muckros Head, Ireland

Cape Town, South Africa

Kalymnos, Greece

Volunteering

CEYE Research Scholar

I helped lead middle school students through dissections of the heart, brain, eyes, and kidneys.

I provided middle schoolers with advice on the high school application process and answered any questions they may have had.

HSMSE Key Club Member

As an active member in HSMSE key club during high school I completed more than 200 hours of volunteer service all around NYC.

Volunteer activities included soup kitchens, community walks, river cleanups, and a variety of other events.

VTP Maths Tutor

During term at Trinity College Dublin I provide grinds each week in maths to a student preparing for his junior cert.

This is part of the Voluntary Tuition Program which helps to provide underpriviledged kids in the Dublin Metro Area with the educational support they need.